| Autor: |
Vanesa Sanchez Garcia de la Torre, Sylvain Merlot, Guillem Rigaill, Sébastien Thomine, Clarisse Majorel-Loulergue, Ludivine Soubigou-Taconnat, Bruno Fogliani, Valerie Burtet-Sarramegna, Dubiel Alfonso Gonzalez, Yohan Pillon, Louise Barreau |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
SSRN Electronic Journal. |
| ISSN: |
1556-5068 |
| Popis: |
The Anthropocene epoch is associated with the spreading of metals in the environment increasing oxidative and genotoxic stress on living organisms. Metalliferous soils such as serpentine can host peculiar plants called hyperaccumulators due to their capacity to accumulate tremendous amount of metal in their shoot (e.g. >0.1% of dry weight for nickel). Once regarded as a curiosity, plants hyperaccumulating metals are now envisioned as an opportunity to remediate metal contaminated soils. The wide phylogenetic distribution of nickel hyperaccumulators (~500 species) suggest that this complex trait evolved multiple times independently from the dysregulation of basic mechanisms involved in metal homeostasis including metal chelation, transport and oxidative stress responses. However, the exact nature of these mechanisms and whether the same molecular mechanisms have been recruited convergently to reach this extreme trait is not known. To address these questions, we developed a comparative cross-species RNA-Seq approach combining differential gene expression analysis and cluster of orthologous group annotation. This analysis reveals candidate orthologous genes encoding convergent function, including the biosynthesis of histidine and flavonoids, involved in nickel hyperaccumulation. Our data also point out that the constitutive high expression of IREG/Ferroportin transporters recurrently emerged as a mechanism involved in nickel hyperaccumulation in a wide diversity of plant families. We further provide genetic evidence in the hyperaccumulator Noccaea caerulescens that IREG/Ferroportin transporters are involved in nickel sequestration in vacuoles. This study paves the way for comparative approaches to identify molecular mechanisms involved in metal hyperaccumulation in a wide diversity of plant species. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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