Capillary enzyme immunoassay with electrochemical detection for determining indole-3-acetic acid in tomato embryos
| Autor: | William R. Heineman, Tongbo Jiang, H. Brian Halsall, Hongying Gao, John L. Caruso |
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| Rok vydání: | 1999 |
| Předmět: |
Detection limit
Flow injection analysis Chromatography medicine.diagnostic_test Chemistry food and beverages Isotope dilution Biochemistry Amperometry Matrix (chemical analysis) chemistry.chemical_compound Immunoassay medicine heterocyclic compounds Indole-3-acetic acid Quantitative analysis (chemistry) |
| Zdroj: | Fresenius' Journal of Analytical Chemistry. 364:170-174 |
| ISSN: | 1432-1130 0937-0633 |
| Popis: | An amperometric immunoassay for indole-3-acetic acid (IAA) in tomato embryos was developed. The antibody against IAA was covalently bound to the chemically modified inner surface of a 22 μL silica capillary. The competitive binding of methylated IAA and an IAA alkaline phosphatase conjugate for a limited amount of antibody was accomplished in the silica capillary in 20 min. The enzymatic product p-aminophenol, produced from p-aminophenyl phosphate, was determined by flow injection analysis with amperometric detection. A detection limit for IAA of 3 pg/μL was obtained. The average concentration of IAA in mature embryos measured by this method is 0.37 ± 0.03 μg/g. Although isotope dilution GC-SIM-MS can achieve the same detection limit, the sample analysis is not feasible in such a complicated matrix and further purification and separation are required. |
| Databáze: | OpenAIRE |
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