Synthesis of Antioxidant Peptides from Melinjo (Gnetum gnemon) Seed Protein Isolated Using Sol-Gel Immobilized Alcalase
| Autor: | Anang Supriyadi, Anadini Aulia Safiera, Tri Agus Siswoyo, Nur Fauziah Matra |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification Chromatography Antioxidant ABTS General Computer Science biology DPPH medicine.medical_treatment 010401 analytical chemistry General Engineering Fast protein liquid chromatography Peptide biology.organism_classification 01 natural sciences Hydrolysate 0104 chemical sciences chemistry.chemical_compound Hydrolysis Biochemistry chemistry 010608 biotechnology medicine Gnetum gnemon General Agricultural and Biological Sciences |
| Zdroj: | International Journal on Advanced Science, Engineering and Information Technology. 7:1315 |
| ISSN: | 2460-6952 2088-5334 |
| DOI: | 10.18517/ijaseit.7.4.936 |
| Popis: | The synthetic of antioxidant peptides from melinjo ( Gnetum gnemon ) seed protein isolated using sol-gel immobilized alcalase was studied, a commercial alcalase was physically entrapped in sol-gel matrices system. Characteristics of protein hydrolyzed were evaluated by the degree of hydrolysis obtained (DH) and the peptide profile by FPLC and SDS-PAGE. The antioxidant activity of protein hydrolyzed was determined using ABTS cation, DPPH, and superoxide radical scavenging assays. The DH values increased very quickly during the second hour followed by a slow increase up to 12 hours. Melinjo seed protein isolated was hydrolyzed about 23% of degree hydrolysis at 50°C after 2 hours of incubation. Similar results were also obtained by FPLC and SDS-PAGE, the peptide profile indicated the sol-gel immobilized alcalase was produced of peptides with low molecular weight from melinjo seed protein isolated. Immobilized alcalase preserved the initial activity over 4 cycles of repeated use in a routine reaction. The ABTS assay, the hydrolysate presented a more than 5 times greater activity than non-hydrolyzed melinjo seed protein isolated. It's confirming the potential use of sol-gel immobilized alcalase in synthetic antioxidant peptides from melinjo seed protein isolated. |
| Databáze: | OpenAIRE |
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