Frequency of Helicobacter pylori hopQI, hopQII and sabA Genes Among Iranian Patients with Gastroduodenal Diseases
| Autor: | Behnam Zamanzad, Nader Bagheri, Heshmat Shahi, Marzieh Sadeghiani, Hedayatollah Shirzad, Reza Khashei, Zahra Sedarat |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_specialty Virulence Chronic gastritis Microbiology Gastroenterology law.invention 03 medical and health sciences 0302 clinical medicine law Internal medicine Medicine Pathogen Genotyping Polymerase chain reaction biology business.industry Cancer Helicobacter pylori medicine.disease biology.organism_classification DNA extraction 030104 developmental biology Infectious Diseases 030211 gastroenterology & hepatology business |
| Zdroj: | Jundishapur Journal of Microbiology. 11 |
| ISSN: | 2008-4161 2008-3645 |
| DOI: | 10.5812/jjm.56017 |
| Popis: | Background: Helicobacter pylori has been recognized as the most common pathogen of human gastroduodenal tract and it has been suggested that adhesins, including HopQ and SabA, are associated with the organism’s virulence. Objectives: The current study aimed at determining the frequency of hopQI, hopQII, and sabA genes among H. pylori isolates from patients with gastroduodenal disorders in Shahrekord, Iran. Methods: Gastric corpus samples were obtained from 150 symptomatic patients admitted to the endoscopy unit at gastroenterology clinic. After DNA extraction from all corpus samples, H. pylori molecular confirmation and genotyping was performed by the polymerase chain reaction (PCR), using specific primers for glmM, 16SrRNA and hopQ, sabA genes, respectively. Results: The hopQI, hopQII, and sabA genes were found in 74 (49.3%), 59 (39.3%), and 43 (28.7%) cases, respectively. The hopQI gene was detected in 75% of patients with gastric cancer (GC), 42.4% with chronic gastritis (CG), and 57.4% with peptic ulcer disease (PUD). The hopQII among patients with GC, CG, and PUD was also detected in 50%, 38.8%, and 39.3%, respectively. Moreover, sabA was diagnosed in 50% of patients with GC, 29.4% with CG, and 26.2% with PUD. Conclusions: No significant association was observed between hopQI, hopQII, and sabA genes with clinical outcomes. |
| Databáze: | OpenAIRE |
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