Increased hydrogen yield and COD removal from starch/glucose based medium by sequential dark and photo-fermentation using Clostridium butyricum and Rhodopseudomonas palustris
| Autor: | Carolina Zampol Lazaro, Patrick C. Hallenbeck, Zeynep Yilmazer Hitit |
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| Rok vydání: | 2017 |
| Předmět: |
Chromatography
biology Renewable Energy Sustainability and the Environment Starch 05 social sciences Energy Engineering and Power Technology 02 engineering and technology Dark fermentation 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification Dilution chemistry.chemical_compound Fuel Technology chemistry Yield (chemistry) 0502 economics and business Fermentation 050207 economics Rhodopseudomonas palustris 0210 nano-technology Clostridium butyricum Hydrogen production |
| Zdroj: | International Journal of Hydrogen Energy. 42:18832-18843 |
| ISSN: | 0360-3199 |
| Popis: | Hydrogen can be produced via dark and photo-fermentation using either single-stage or two-stage processes. The advantage of a two-stage system is that it is possible to separately optimize and control culture conditions for the dark and photo-fermentative bacteria. In the present study a mixture of starch and glucose was used as carbon source for the dark fermentation step. Response surface methodology (RSM) with a Box-Behnken design (BBD) was applied to the photo-fermentation stage for the optimization of key parameters: inoculum concentration (Rhodopseudomonas palustris), substrate concentration (dark fermentation effluent (DFE)) and pH. In this sequential two-stage system, the highest overall hydrogen yield (8.3 ± 0.1 mmol H2/gCOD), overall hydrogen production (1.62 mmol) and the photo fermentation yield (7.21 ± 0.2 mmol H2/gCOD) were achieved at inoculum of 9 mL (1.64 ± 0.7 × 108 cells), a DFE dilution of 2.5× and a pH of 7.5, which were center points of the design. 97% COD removal was achieved at the highest dilution of DFE (lowest concentration of carbon source) at pH 6.5. |
| Databáze: | OpenAIRE |
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