Isolation and multiplex analysis of six polymorphic microsatellites in the Antarctic notothenioid fish, Trematomus newnesi
| Autor: | F. A. M. Volckaert, Philippe Koubbi, A. P. Van de Putte, Bart Hellemans, J. K. J. Van Houdt |
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| Rok vydání: | 2006 |
| Předmět: |
Genetics
education.field_of_study Ecology fungi Population Locus (genetics) Biology biology.organism_classification Biochemistry Genetic analysis General Biochemistry Genetics and Molecular Biology Loss of heterozygosity Evolutionary biology Microsatellite Nototheniidae Allele education Microsatellite enrichment |
| Zdroj: | Molecular Ecology Notes. 6:157-159 |
| ISSN: | 1471-8286 1471-8278 |
| Popis: | Six polymorphic microsatellites were isolated from the dusky notothen, Trematomus newnesi (Boulenger 1902), using a microsatellite enrichment protocol and selective hybridization with di- tetra- and penta-repeat probes. The loci were screened in 48 individuals captured in the Southern Ocean (coastal zone of Terre-Adelie), revealing eight to 22 alleles per locus and an observed heterozygosity ranging from 0.70 to 0.92. These microsatellite markers provide a tool to study the relationship between the various morphs observed in this species and can be used for population genetic analysis and biodiversity studies. |
| Databáze: | OpenAIRE |
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