Comparison of the Pharmacological Properties of Rat NaV1.8 with Rat NaV1.2a and Human NaV1.5 Voltage-Gated Sodium Channel Subtypes Using a Membrane Potential Sensitive Dye and FLIPRR

Autor:	N Mai, J A M Smith, R G Vickery, R Chang, J Martin, E Kaufman, D. Marquess, Jeremy Hembrador, S.M. Amagasu, M D O'Keefe, C Gee
Rok vydání:	2004
Předmět:	Pharmacology Membrane potential Voltage clamp Sodium channel Clinical Biochemistry Depolarization Cell Biology NAV1.5 Voltage-Gated Sodium Channel chemistry.chemical_compound Endocrinology chemistry Tetrodotoxin Biophysics Patch clamp Veratridine
Zdroj:	Receptors and Channels. 10:11-23
ISSN:	1543-5334 1060-6823
DOI:	10.3109/10606820490270410
Popis:	A novel, membrane potential sensitive dye and a fluorescence imaging plate reader (FLIPR) have been used to characterize the pharmacological properties of rat Na(v)1.8 voltage-gated sodium channels (VGSC) in parallel with rat Na(v)1.2a and human Na(v)1.5 VGSC subtypes, respectively. The sensitivity of recombinant Na(v)1.2a-CHO, Na(v)1.5-293-EBNA, and Na(v)1.8-F-11 cells to VGSC activators was subtype dependent. Veratridine evoked depolarization of Na(v)1.2a-CHO and Na(v)1.5-293-EBNA cells with pEC(50) values of 4.78 +/- 0.13 and 4.84 +/- 0.12, respectively (n = 3), but had negligible effect on Na(v)1.8-F-11 cells (pEC(50) or =20-fold, in contrast to a
Databáze:	OpenAIRE
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