96 GRANULOCYTE–MACROPHAGE COLONY-STIMULATING FACTOR INCREASES DEVELOPMENTAL POTENTIAL OF PORCINE EMBRYOS IN VITRO
| Autor: | Kiho Lee, Clifton N. Murphy, Randall S. Prather, Lee D. Spate, Jennifer M. Teson |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Zygote
Embryo culture Embryo Anatomy Reproductive technology Biology Cryopreservation Andrology Endocrinology medicine.anatomical_structure Reproductive Medicine embryonic structures Genetics medicine Somatic cell nuclear transfer Animal Science and Zoology Blastocyst Molecular Biology Fertilisation Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 24:160 |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/rdv24n1ab96 |
| Popis: | There have been significant improvements in the culture of porcine embryos in vitro; however, it is still suboptimal. Improvements in porcine embryo culture would benefit utilisation of porcine embryos for a variety of purposes. Granulocyte-macrophage colony-stimulating factor (GM-CSF) is known to be expressed in the female reproductive tract and the level of its expression is high between conception and implantation. Previous studies show supplementing GM-CSF in embryo culture promotes embryonic development in human and bovine embryos. The aim of this study was to investigate the effect of GM-CSF on the culture of porcine embryos derived from somatic cell nuclear transfer (SCNT) and IVF. Different concentrations of recombinant porcine GM-CSF (0, 2, 10 ng mL–1) were introduced into Porcine Zygote Medium 3 from Day 1 to 6. Frequencies of cleaved embryos and blastocyst formation were recorded and analysed by using ANOVA following arcsin transformation. Total cell number in blastocysts from each group were counted and compared by using the Student's t-test. Differences at P |
| Databáze: | OpenAIRE |
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