Synthesis, characterization and cytotoxicity of the Eugenia brejoensis essential oil inclusion complex with β-cyclodextrin

Autor: Maria Tereza dos Santos Correia, Rayane Cristine Santos da Silva, Bianca Galúcio Pereira Araújo, Sophie Fourmentin, Alexandre Gomes da Silva, Keicyanne Fernanda Lessa dos Anjos, Márcio Akio Ootan, Giovanna Machado, Nataly Amorim de Santana, Márcia Vanusa da Silva
Rok vydání: 2020
Předmět:
Zdroj: Journal of Drug Delivery Science and Technology. 60:101876
ISSN: 1773-2247
Popis: This study investigated for the first time the effect of Eugenia brejoensis essential oil (EB) and its inclusion complex (IC) with β-cyclodextrin (β-CD) on Hela and J774 cell lines. Solid IC was prepared by the coprecipitation method and characterized by several analytical methods: differential scanning calorimetry (DSC), thermogravimetry analysis (TGA), X-ray diffraction (XRD), RAMAN spectroscopy and scanning electron microscopy (SEM). The loading capacity (LC) was determined. The formation constant of β-CD/caryophyllene (the main constituent of EB essential oil) as well as the ability of β-CD to retain EB and caryophyllene were evaluated by static headspace gas chromatography (SH-GC) in aqueous solution. Finally, the cytotoxicity of EB and β-CD/EB IC was determined on Hela and J774 cell lines. We clearly demonstrated the formation of an inclusion complex between EB EO and β-CD in solid state and aqueous solution. Indeed, the amount of EB included in the solid IC was equal to 4.7 μg of essential oil/mg of solid complex and the obtained formation constant between β-CD and β-(E)-caryophyllene, the main constituent of the EB, was as high as 28460 M−1. Moreover we could observe a greater selectivity of the β-CD/EB IC for the tumor lineage. Therefore, β-CD/EB IC could be a promising agent with potential application in anticancer drug development.
Databáze: OpenAIRE
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