Microplate-Based Method for High-Throughput Screening (HTS) of Chromatographic Conditions Studies for Recombinant Protein Purification
| Autor: | Thayana A. Cruz, Rimenys J. Carvalho |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chromatography Computer science High-throughput screening Design of experiments 010401 analytical chemistry 01 natural sciences 0104 chemical sciences law.invention 03 medical and health sciences 030104 developmental biology law Protein purification High-Throughput Screening Assays Cost evaluation Recombinant DNA Scale down |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493973118 |
| DOI: | 10.1007/978-1-4939-7312-5_17 |
| Popis: | High-throughput screening (HTS) systems have emerged as important tools to provide fast and low cost evaluation of several conditions at once since it requires small quantities of material and sample volumes. These characteristics are extremely valuable for experiments with large number of variables enabling the application of design of experiments (DoE) strategies or simple experimental planning approaches. Once, the capacity of HTS systems to mimic chromatographic purification steps was established, several studies were performed successfully including scale down purification. Here, we propose a method for studying different purification conditions that can be used for any recombinant protein, including complex and glycosylated proteins, using low binding filter microplates. |
| Databáze: | OpenAIRE |
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