Simultaneous HPLC Separation of Diastereoisomers of the Oxazepam Glucuronide and Oxazepam. Application to the Study of Urinary Excretion
| Autor: | R. V. Mawa, M. C. Gagnieu, J. J. Vallon, Michelle Petit-Ramel, Denis Grancher |
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| Rok vydání: | 1995 |
| Předmět: |
Chromatography
Chemistry Metabolite Biochemistry (medical) Clinical Biochemistry Diastereomer Urine Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Urinary excretion Oxazepam Electrochemistry medicine Quantitative analysis (chemistry) Diazepam Spectroscopy medicine.drug |
| Zdroj: | Analytical Letters. 28:1387-1404 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1080/00032719508006402 |
| Popis: | The authors present a simple liquid chromatographic method which enables direct separation of S(+) and R(−) oxazepam glucuronides and free oxazepam as well as diazepam (internal standard) in a single run. Metabolites and oxazepam are quantified by direct injection of diluted urine. Application to the study of urinary excretion in sheep revealed a S/R ratio of 6.37 which is one of the highest ever observed among various animal species. The percentage eliminated as refered to administrated oxazepam was found to be near 35% which is particularly low compared to other species (60 to 80%). |
| Databáze: | OpenAIRE |
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