Carbon-specific detection of 2H enrichment in long chain fatty acids by 13C-NMR: potential application to studies of fatty acid metabolism
| Autor: | F Mendy, B Descomps, Peter Haycock, Steven Williams, Z Y Chen, Stephen C. Cunnane |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
Chromatography Fatty acid metabolism Linolenic acid Organic Chemistry Fatty acid Cell Biology Nuclear magnetic resonance spectroscopy Carbon-13 NMR Mass spectrometry Biochemistry chemistry.chemical_compound chemistry Organic chemistry Dihomo-gamma-linolenic Acid Molecular Biology Fatty acid synthesis |
| Zdroj: | Chemistry and Physics of Lipids. 63:259-263 |
| ISSN: | 0009-3084 |
| Popis: | We have investigated the possibility of using 13C-NMR spectroscopy to detect carbon-specific 2H enrichment in dihomo-gamma-linolenic acid and in rat liver lipids. Dihomo-gamma-linolenic acid was chemically synthesized with 98% 2H enrichment specifically at C-18 and C-19 as verified by mass spectrometry and 2H-NMR. Due to a tenfold increase in spin-lattice relaxation time, the C-18 and C-19 peaks normally present at 31.38 and 22.43 ppm, respectively, were not visible in 13C-NMR spectra of [18,18,19,19-2H]-dihomo-gamma-linolenic acid acquired at 1-s pulse repetition rates. However, at 20-s pulse repetition rates, these peaks were both visible as small quintets. This observation was applied to study the effect of deuteration on 13C-NMR peak heights of rat liver fatty acids after consumption of deuterated water. At 1-s pulse repetition rates, 2H enrichment in rat liver total lipids suppressed 13C-NMR signals from acyl carbons except at specific carbons where 2H could not be inserted (mostly in the methyl terminal region and doubly allylic carbons of polyenes). This methodology may therefore have application in the carbon-by-carbon study of fatty acid synthesis and/or degradation. |
| Databáze: | OpenAIRE |
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