Analytical studies and preparative separation of ansamitocins and related antibiotics using high-performance liquid chromatography

Autor:	Konomi Haibara, Mitsuko Asai, Motowo Izawa
Rok vydání:	1980
Předmět:	Chromatography Aqueous solution Extraction (chemistry) Ethyl acetate General Chemistry General Medicine High-performance liquid chromatography Solvent chemistry.chemical_compound chemistry Yield (chemistry) Drug Discovery Methanol Chromatography column
Zdroj:	Chemical and Pharmaceutical Bulletin. 28:789-794
ISSN:	1347-5223 0009-2363
DOI:	10.1248/cpb.28.789
Popis:	Ansamitocins and related compounds were isolated from the culture broth of Nocardia sp. 1) The total amount of the antibiotics, after extraction with ethyl acetate from the culture broth, was determined by high-performance liquid chromatography on a μ Porasil column with a solvent mixture of methylene chloride-methanol (20 : 1). 2) Each component in the mixture was quantitatively analyzed on a μ Bondapak column with 69% aqueous methanol. 3) Preparative separation of up to 5 g per cartridge column was carried out in good yield (90%) using a PrepLC/system 500 (Waters) and a column packing of Kieselgel 60 (E. Merck). The purities of the ASM obtained were above 95%.
Databáze:	OpenAIRE
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