Expression inE. coli of the cloned cDNA for the major antigen of foot and mouth disease virus Asia 1 63/72

Autor: V S V Suryanarayana, J D Padayatty, B U Rao
Rok vydání: 1986
Předmět:
Zdroj: Journal of Genetics. 65:19-30
ISSN: 0973-7731
0022-1333
DOI: 10.1007/bf02923532
Popis: Double stranded cDNA for the foot and mouth disease virus was prepared, restricted with BamH 1 or ligated to linkers with BamH 1 sticky ends and cloned in BamHI site in the expression vector,pUR222. The cDNA was also cloned at the Pst l site in the same vector by the dC/dG tailing method. They were transferred into E. coli to give colourless colonies in the presence of the dye, X-gal. Many of them showed positive signal on hybridization with $^{32}P$-labelled viral RNA. The middle BomH 1 fragment of the cDNA is known to carry the gene for the major antigen and some non-structural proteins. The clones carrying the recombinant DNA produced proteins which cross-reacted with the antibodies generated against the structural proteins of the virus in an enzyme linked immunosorbent assay, indicating that the cDNA of the major antigen is expressed in the cloned cell.
Databáze: OpenAIRE
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