Removal of DNA from a Protein Solution with Cross‐Linked Poly(Ethyleneimine) Spherical Particles
| Autor: | Hiroshi Mizokami, Masashi Kunitake, Kouchi Matsumoto, Nagi Obaru, Chuichi Hirayama, Masayo Sakata |
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| Rok vydání: | 2003 |
| Předmět: |
Chromatography
biology Chemistry Clinical Biochemistry technology industry and agriculture Serum albumin Ethyleneimine Pharmaceutical Science macromolecular substances Biochemistry Analytical Chemistry Suspension (chemistry) Adsorption Ionic strength Nucleic acid biology.protein Bovine serum albumin Selectivity |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 26:231-246 |
| ISSN: | 1520-572X 1082-6076 |
| Popis: | To remove nucleic acids from a solution of a cell product used as a drug, water‐insoluble poly(ethyleneimine) (PEI) spherical particles were prepared by suspension cross‐linking with PEI and chloromethyloxirane. The PEI content of the particles was easily adjusted by changing the PEI ratio and the CMO ratio in the cross‐linking. The cross‐linked PEI particles, which had diameters of 44 to 105 µm and PEI contents of 50 to 90 unit mol%, were used as adsorbents. The adsorption of DNA and cell products to the adsorbents was determined using a batchwise method. The larger the PEI content of the adsorbent, the larger the DNA‐adsorbing activity of the adsorbent. The apparent dissociation constant between the DNA (purified DNA from salmon spermary) and the adsorbent, decreased from 8.5 × 10−8 to 9.5 × 10−10 M with an increase in PEI ratio from 50 to 90 unit mol% under physiological conditions (ionic strength (μ) = 0.17, pH 7.2). On the other hand, the adsorbing activity of bovine serum albumin also incre... |
| Databáze: | OpenAIRE |
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