| Popis: |
An High Pressure Liquid Chromatography method for analysis of Solvent Yellow 33 purity is investigated, developed and tested. The HPLC method is found to be more accurate and more precise than earlier methods. In addition, the automated HPLC system is less time intensive and therefore more cost efficient. The method involves analysis in methanol solution on a reverse phase C-18 Column using a UV detector set at 429m. The current method for solvent yellow 33 (SY-33) purity analysis stipulated in DOD-D-51485 is in need of revision. This method is thought inadequate because the dilution scheme is labor intensive and time consuming. In addition, the analytical results often vary widely from one laboratory to another, and from one HPLC operator to another. The precision of the current method is typically unacceptable. The following report presents the results of an intralaboratory evaluation of a new liquid chromatographic method for the purity assessment of solvent yellow 33 (CI 47000). The report also presents a comprehensive explanation of the laboratory techniques involved in the sample preparation for dye purity assessment. |
