Studies on methanol metabolism. II. Further studies of vitamin B12 production by methanol utilizing bacterium, Klebsiella sp. No. 101

Autor:	Takuo Yano, Tadashi Kamikubo, Naomichi Nishio
Rok vydání:	1975
Předmět:	Ammonium sulfate Ammonium nitrate Cystine chemistry.chemical_element Phenylalanine Bacterial growth Calcium General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound chemistry Biochemistry Urea Vitamin B12 Food science General Agricultural and Biological Sciences
Zdroj:	Agricultural and Biological Chemistry. 39:207-213
ISSN:	1881-1280 0002-1369
DOI:	10.1271/bbb1961.39.207
Popis:	The accumulation of vitamin B12 by Klebsiella sp. No. 101 grown on methanol was investigated furthermore. Addition of urea together with ammonium sulfate or ammonium nitrate was more effective than other nitrogen sources tested. Simultaneous addition of metal ions such as ferrous, calcium and manganese, and addition of amino acids such as alanine, valine, leucine, cysteine, cystine, phenylalanine, tyrosine and proline promoted the bacterial growth and vitamin B12 production. Optimal pH for vitamin B12 production was near 7.0. pH adjustment and methanol feeding were effective for the growth and the vitamin formation. Vitamin B12 production was decreased by addition of CN–B12 to the medium at the concentrations more than 100 μg per liter.
Databáze:	OpenAIRE
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