Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds
| Autor: | Bekir San, Man Zhou, Fangyuan Gao, Junming Zhao, Dayong Li, Hong Luo, Shuangrong Yuan, Zhigang Li, Qian Hu, Ning Yuan, Haiyan Jia |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Agrostis stolonifera biology Transgene fungi food and beverages Genetically modified crops biology.organism_classification 01 natural sciences Gene gun 03 medical and health sciences Transformation (genetics) Horticulture 030104 developmental biology Callus Northern blot Gene 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071603550 |
| DOI: | 10.1007/978-1-0716-0356-7_13 |
| Popis: | We describe a protocol for the establishment and preparation of creeping bentgrass (Agrostis stolonifera L.) cultivar "Penn A-4" embryonic calli, biolistic transformation, selection, and regeneration of transgenic plants. The embryonic callus is initiated from mature seeds, maintained by visual selection under the dissecting microscope and subjected to bombardment with plasmid DNA containing a bialaphos-resistance (bar) gene. PCR, Southern, and Northern blot analyses are used to confirm the transgene integration and expression. |
| Databáze: | OpenAIRE |
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