Cutinases obtained from filamentous fungi: comparison of screening methods
| Autor: | Erlide Prieto-Correa, Carlos Jiménez-Junca, Hugo Rueda-Rueda |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Fusarium Cutinase Mucor 0303 health sciences biology Chemistry Cutinase activity General Engineering biology.organism_classification Penicillium chrysogenum 01 natural sciences Esterase 03 medical and health sciences 010608 biotechnology Penicillium Screening method Food science 030304 developmental biology |
| Zdroj: | DYNA. 87:183-190 |
| ISSN: | 2346-2183 0012-7353 |
| DOI: | 10.15446/dyna.v87n214.83737 |
| Popis: | espanolLas cutinasas son secretadas por hongos filamentosos que hidrolizan polimeros. Sin embargo, pocos metodos de seleccion estan disponibles. En este trabajo, tres metodos fueron estudiados, usando 33 cepas de hongos filamentosos aislados de banana rachis con alto potencial para producir cutinasas. En el primer metodo, las cepas fueron crecidas en medio mineral Czapec-Dox que contenia aceite de linasa, encontrandose que seis cepas de los generos Fusarium, Penicillium and Mucor podrian tener actividad cutinasa. El segundo metodo consistio en evaluar las cepas con triacetina en rodamina, indicando que cepas tenian propiedades esterasas. Finalmente, las cepas fueron sometidas a fermentacion con aceite de linasa; actividad lipolitica y cutinolitica fue determinada. Las especies identificadas como las mejores productoras de cutinasas fueron Fusarium fujikuroi y Penicillium chrysogenum, obteniendose 2 cutinasas extracelulares con actividades de 33.5 U/mL y 39.4 U/mL respectivamente. Las cutinasas fueron confirmadas por degradacion de cutina de tomate a traves de FTIR EnglishCutinases are secreted by filamentous fungi that and hydrolyze polymers. However, few selection methods for cutinases are available. Here, we studied three screening methods using 33 strains of filamentous fungi isolated from banana rachis with high potential to produce cutinases. In the first method, strains were grown in Czapec-Dox mineral medium containing flaxseed oil. We note that six strains of the genera Fusarium, Penicillium, and Mucor had cutinase activity. The second method evaluated strains with triacetin in rhodamine B, which indicated what strains had esterase property. Finally, strains were subjected to fermentation with flaxseed oil; lipolytic and cutinolytic activity were determined. The species identified as the best producers of cutinases were Fusarium fujikuroi and Penicillium chrysogenum, and we obtained two extracellular cutinases with activities of 33.5 U/mL and 39.4 U/mL respectively. Cutinase was confirmed via degradation of tomato cutin through FTIR |
| Databáze: | OpenAIRE |
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