| Popis: |
Specific method for direct radioimmunoassay of IRP and IRI separately in human plasma has been described. The method is used for extraction of total insulin and separation of IRP from IRI by paper chromatography to be assayed separately. The separation of the two components are indentified and confirmed by column chromatography, paper chromatography and U.V. spectral analysis in comparison with the standard compounds. 134 plasma samples of different cases were, investigated for determination of IRI, IRP and IRT, of which 39 normals, 16 normal obes, 21 juvinil diabetes, 18 adult oncet diabetes, 10 recent adult diabetes, 12 hypothroid and 18 bilharzial hepatosplenomegaly to evaluate the levels of the test in comparison with blood sugar concentration. |
