Recovery of functionally active trypsin inhibitor (SBTI) and lipoxygenase (LOX) from soymilk via multimodal technique
| Autor: | Tirunagari Harini, Shalini Basetty, Nitin W. Fadnavis, Kuna Lavanya, Lakshmi Swarnalatha Jasti |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Polymers and Plastics
General Chemical Engineering Trypsin inhibitor 01 natural sciences Biochemistry Lipoxygenase chemistry.chemical_compound 0404 agricultural biotechnology Column chromatography Materials Chemistry Environmental Chemistry Bovine serum albumin chemistry.chemical_classification Diethylamine Chromatography Kunitz STI protease inhibitor biology 010401 analytical chemistry 04 agricultural and veterinary sciences General Chemistry Polymer 040401 food science 0104 chemical sciences chemistry Yield (chemistry) biology.protein |
| Zdroj: | Reactive and Functional Polymers. 121:45-50 |
| ISSN: | 1381-5148 |
| DOI: | 10.1016/j.reactfunctpolym.2017.10.009 |
| Popis: | Commercially available epoxy activated polymers Immobeads and Dilbeads were functionalized to 1,2-amino alcohol derivatives and screened for binding and release of soybean trypsin inhibitor (SBTI) and lipoxygenase (LOX) in soymilk. Moderately hydrophobic polymer DILBEAD-VWR functionalized with diethylamine (DIL-DEA) was found to bind SBTI and LOX (3.5 ± 0.3 mg·g− 1) without causing protein denaturation. Bound proteins were selectively released by changing the pH of eluting buffer. LOX was obtained in 70 ± 5% overall yield with a purification factor of 5 ± 0.5. The eluent containing SBTI was treated with bovine serum albumin coated polymer (Immobead 350) to obtain SBTI in 61% overall yield and a purification factor of 13.5. The recovered DIL-DEA polymer as well as BSA coated polymer were recycled at least 10 times without any appreciable change in performance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect