SAT0252 DNASE serum and polyclonal IGG activity in patients with axial and peripheral spondyloarthritides
| Autor: | A.V. Kundzer, M.V. Volkava |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
biology business.industry Immunology Autoantibody Likelihood ratios in diagnostic testing General Biochemistry Genetics and Molecular Biology Abzyme Endocrinology Rheumatology Quartile Affinity chromatography Polyclonal antibodies Internal medicine biology.protein Immunology and Allergy Medicine Antibody Protein A business |
| Zdroj: | Annals of the Rheumatic Diseases. 71:557.1-557 |
| ISSN: | 1468-2060 0003-4967 |
| DOI: | 10.1136/annrheumdis-2012-eular.3199 |
| Popis: | Background Catalytic deoxyribonuclease (DNAse) autoantibodies have been demonstrated in patients with autoimmune and infectious diseases, but conclusive data about DNAse activity of antibodies in patients with spondyloarthritides (SpA) are lacking. Recently the assessment of catalytic serum activity is used for diagnosis of many diseases. The assessment of DNAse serum activity is not used for spondyloarthritides diagnosis. The aim of the study is to assess DNAse abzyme activity of polyclonal IgG and DNAse serum activity in patients with SpA for next elaboration of differentiation criteria for these diseases. Objectives The 125 patients with peripheral SpA, 51 patients with axial SpA and 69 healthy persons were examined. All patients were fulfilled ASAS criteria for peripheral and axial SpA (1). Methods The samples of sera and polyclonal IgG isolated from patients and healthy persons were investigated. IgG were purified from the sera by combined method of affinity chromatography on protein A column. The experiments, confirming that abzyme activity is the essential quality of polyclonal IgG, were performed. The methods for DNAse activity assessment relied upon rivanol capacity to form a clot with DNA reversely proportional to this acid depolymerisation on the action of DNAse. Results The levels of serum DNAse activity in patients with peripheral SpA (Me 4,0 units (min 3,0 max 5,0) (lower quartile 3,0 upper quartile 4,0)) and axial SpA (Me 2,0 units (min 1,0 max 4,0) (lower quartile 1,0 upper quartile 2,0)) were significantly (p |
| Databáze: | OpenAIRE |
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