Studies of mammalian chromosome replication

Autor:	Frances E. Arrighi, Yun Fai Lau
Rok vydání:	1980
Předmět:	Genetics Polytene chromosome Chromothripsis Control of chromosome duplication Differential staining Eukaryotic DNA replication Biology Molecular Biology Chromosome 22 Eukaryotic chromosome structure Metaphase Genetics (clinical)
Zdroj:	Cytogenetic and Genome Research. 27:176-183
ISSN:	1424-859X 1424-8581
DOI:	10.1159/000131479
Popis:	The patterns of differential staining based on the effects of BrdU-substitution in chromosomal DNA have been examined in both metaphase chromosomes and prematurely condensed chromosomes (PCC) of interphase Chinese hamster cells. Results indicate that differential staining may be obtained in chromosomes from all stages of the cell cycle and correspond to the semi-conservative mode of DNA replication. Such fidelity of differential staining in both interphase and metaphase chromosomes suggests that components essential for induction of differential staining are present throughout the cell cycle and chromosomes may contain similar structures and organization throughout the cycle. Differential staining of metaphase chromosomes based on the effects of substitution of BrdU for thymidine in DNA has been extensively employed for various mutagen-carcinogen assays (Bloom and Hsu, 1975; Perry and Evans, 1975; Allen and Latt, 1976; Wolff, 1977, and Carrano et al., 1978) and studies on DNA replication and
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6f248b3b71413b7a3260d988dc7e2142 https://doi.org/10.1159/000131479 Zobrazit plný text záznamu