In vivo Genotoxicity Assessment of Matrine and the Water Extract of Sophorae Radix Using a Comet Assay
| Autor: | Hyoung-woon Jeon, Ji-Yeon Lee, Myoungjun Kim, Seongmoo Heo, Young-Shin Chung |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Journal of Food Hygiene and Safety. 36:118-123 |
| ISSN: | 2465-9223 1229-1153 |
| DOI: | 10.13103/jfhs.2021.36.2.118 |
| Popis: | The genotoxic potentials of the water extract of Sophorae Radix (WSR) and matrine as a major compound were investigated in murine hepatocytes and blood cells using single-cell gel electrophoresis. According to the test guidelines of OECD TG 489, an in vivo alkaline Comet assay was performed using male mice. The treatment doses of the WSR were 1,000 and 2,000 mg/kg body weight to include the limiting dose. The three test doses for matrine were 50, 100, and 200 mg/kg, and the maximum dose was estimated from a dose range-finding test. The negative control and test articles were administered three times by oral gavage, and the positive control (ethyl methanesulfonate, EMS) was administered twice. Within 3-4 h after the last treatment, the liver tissue and blood were collected, and single-cell electrophoresis was performed under alkaline conditions. The in vivo Comet assay showed no changes in body weights during the treatments with the test articles. The WSR and matrine did not induce significant DNA damage in the liver and blood cells of mice compared to the vehicle treatment. In conclusion, matrine and the WSR may not have genotoxic potential. |
| Databáze: | OpenAIRE |
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