Determination of physiological noble metals in human urine using liquid-liquid extraction and Zeeman electrothermal atomic absorption spectrometry

Autor: Jutta Begerow, Martina Turfeid, Lothar Dunemann
Rok vydání: 1997
Předmět:
Zdroj: Analytica Chimica Acta. 340:277-283
ISSN: 0003-2670
DOI: 10.1016/s0003-2670(96)00486-2
Popis: An extremely sensitive, reliable and simple procedure is described for the determination of physiological palladium, platinum and gold in human urine. The urine samples were adjusted to pH 4 (Pd, Au) or pH 5 (Pt), followed by conversion of the analytes to their pyrrolidinedithiocarbamate complexes. These complexes were separated from the matrix by liquid-liquid extraction into 4-methyl-2-pentanone resulting in a 25-fold enrichment. Determination was by electrothermal atomic absorption spectrometry (ET-AAS) using longitudinal inverse alternating current Zeeman-effect background correction. The limits of detection calculated from three standard deviations of the blank values were 20 ng l−1 for Pd and Au and 70 ng l−1 Pt. Within-day precision (n = 10, 5 μg l−1) ranged 5.2%–7.7%. The procedure is successfully applied to determine urinary palladium, platinum and gold in nine unexposed persons. Palladium levels in urine ranged < 20–80 ng l−1 (arithmetical mean=38.7 ng l−1), while gold levels ranged < 20–130 ng l−1 (36.0 ng l−1). Physiological platinum levels in urine were all < 70 ng l−1. The accuracy of the procedure was checked by analyzing a series of urine samples by a second independent method (magnetic sector field inductively-coupled plasma-mass spectrometry) in combination with UV photolysis.
Databáze: OpenAIRE
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