| Autor: |
J. Bereiter-Hahn, B. Keller-Stanislawski, Ana Ramirez-Bosca, C. Theilig, August Bernd, F. E. Görmar, Hans Holzmann, N. Rietbrock |
| Rok vydání: |
1993 |
| Předmět: |
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| Zdroj: |
Cell and Tissue Culture Models in Dermatological Research ISBN: 9783642778193 |
| Popis: |
The influence of nicotine on cell differentiation was investigated. Cultures of human keratinocytes (HaCaT) were stained with an antibody against pan- keratin. They showed a more ordered keratin pattern with a nicotine concentration starting at 0.1 μg/ml in contrast to the control cultures. The expression of the keratins 1 and 10/11, which are regarded as differentiation markers [13, 15], increased after treatment with low nicotine concentrations (0.1–10 mg/ml medium). The number of “cornified envelopes” increased at a concentration of 0.1–100 μg nicotine/ml medium. In two-dimensional SDS-gel electrophoresis, cytokeratin 1 appeared at a nicotine concentration of 1 μg/ml medium. This was not found in parallel controls. This demonstrates that low concentrations of nicotine have a supporting influence on the keratinization and cornification of HaCaT keratinocytes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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