| Autor: |
M.L. Wilhelm, F.X. Wilhelm, T. Heyman, Mansour Boutabout |
| Rok vydání: |
1999 |
| Předmět: |
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| Zdroj: |
Journal of Molecular Biology. 288:505-510 |
| ISSN: |
0022-2836 |
| DOI: |
10.1006/jmbi.1999.2723 |
| Popis: |
Replication of the yeast Ty1 retrotransposon occurs by a mechanism similar to that of retroviruses. According to the current model of retroviral reverse transcription, two strand transfers (the so-called minus-strand and plus-strand strong-stop DNA transfers) are required to produce full-length preintegrative DNA. Because two genomic RNA molecules are packaged inside the viral particles, the strand transfers can be either intra- or intermolecular. To study the mode of transfer of minus-strand strong-stop DNA during reverse transcription of the yeast Ty1 retrotransposon, we have analyzed the cDNA products that accumulate in the cytoplasmic virus-like particles of yeast cells harboring two marked Ty1 elements. Our results indicate that Ty1 minus-strand transfer occurs in a random manner with approximately similar frequencies of intra- and intermolecular transfer. It has been observed recently that intra- and intermolecular minus-strand transfer occur at similar frequencies during replication of a complex retrovirus such as HIV-1. These results together with the observation that genetic recombination occurs with a high frequency during minus-strand synthesis suggest that both packaged RNA molecules are needed for the synthesis of one minus-strand DNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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