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The process of androgenesis is unique, in that haploid embryos arise from single gametophytic cells. Clear evidence for the unicellular origin of somatic embryos is lacking for most regeneration systems (Fransz and Schel 1991); therefore, totipotent microspores offer a potentially useful subject for both basic and applied studies. Although anther culture can be an efficient method of producing doubled haploid lines for breeding applications, culture of isolated microspores provides a more useful Experimental system, allowing direct manipulation and observation during all stages of development. |
