Effectiveness of the Sleep Enhancement by Green Romaine Lettuce (Lactuca sativa) in a Rodent Model
| Autor: | Hyung Joo Suh, Ki Bae Hong, Hyeon Son Choi, Kyungae Jo, Hyo Won Kim |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pharmacology medicine.medical_specialty Pentobarbital Chemistry Lactucin Antagonist Pharmaceutical Science General Medicine Sleep in non-human animals Non-rapid eye movement sleep 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Endocrinology Flumazenil 030220 oncology & carcinogenesis Internal medicine polycyclic compounds medicine Insomnia medicine.symptom medicine.drug Lactucopicrin |
| Zdroj: | Biological and Pharmaceutical Bulletin. 42:1726-1732 |
| ISSN: | 1347-5215 0918-6158 |
| Popis: | This study was conducted to investigate the effects of the extracts of green romaine lettuce (GRE) on sleep enhancement. GRE contains 1071.7 and 199.2 µg/g of extracts of lactucin and lactucopicrin, respectively, known as sleep enhancement substances. When 100 mg/kg of GRE was administered orally, sleep latency and duration time were significantly increased compared to controls (p < 0.05). Rapid eye movement (REM) sleep decreased with 100 mg/kg of GRE administration and non-REM (NREM) sleep also increased. There was no significant difference between REM and NREM among the oral GRE administration groups receiving 100, 120, and 160 mg/kg GRE. In the caffeine-induced insomnia model, total sleep time was significantly increased by 100 mg/kg GRE administration compared to the caffeine-treated group (p < 0.05). In addition, GRE inhibited the binding of [3H]-flumazenil in a concentration-dependent manner, and affinity of both lactucin and lactucopicrin to gamma-aminobutyric acid (GABA)A-benzodiazepine (BDZ) receptor was 80.7% and 55.9%, respectively. Finally, in the pentobarbital-induced sleep mouse model, the sleep enhancement effect of GRE was inhibited by flumazenil, an antagonist of BDZ. Thus, these results demonstrate that GRE acts via a GABAergic mechanism to promote sleep in a rodent model. |
| Databáze: | OpenAIRE |
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