Polysulfone Hydrogel Nanocomposite Alkaline Phosphatase Biosensor for the Detection of Vanadium
| Autor: | Priscilla G. L. Baker, Francis N. Muya, Emmanuel I. Iwuoha |
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| Rok vydání: | 2020 |
| Předmět: |
Detection limit
Materials science Calibration curve technology industry and agriculture Vanadium chemistry.chemical_element macromolecular substances 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Amperometry 0104 chemical sciences Dielectric spectroscopy Linear range chemistry Electrochemistry Cyclic voltammetry 0210 nano-technology Biosensor Nuclear chemistry |
| Zdroj: | Electrocatalysis. 11:374-382 |
| ISSN: | 1868-5994 1868-2529 |
| DOI: | 10.1007/s12678-020-00592-0 |
| Popis: | Several biosensors based on the immobilization of alkaline phosphatase (ALP) at different transducers have been reported in literature. In this work, a gold electrode covalently modified with a hydrogel (Au-HGL) was used as a platform for the immobilization of ALP enzyme to produce Au-HGL/ALP biosensor. The biosensor construction was evaluated by Raman spectroscopy, cyclic voltammetry (CV), and atomic force microscopy (AFM). The analytical performance of the biosensor was assessed by square wave voltammetry (SWV), electrochemical impedance spectroscopy (EIS), and amperometry. The calibration curve displayed a dynamic linear range of 0.5–30 μM with a detection limit (LOD) and quantification limit (LOQ) of 0.23 μM and 0.76 μM, respectively. Determination of vanadium in commercial Centrum® tablet using the proposed biosensor was satisfactory when compared to other analytical methods. The proposed Au-HGL/ALP biosensor exhibited high sensitivity to vanadium in an aqueous medium with a good reproducibility (n = 4) and a relative standard deviation (RSD) of 8.0%. |
| Databáze: | OpenAIRE |
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