DNA content of nuclei ofActinidia deliciosavar.deliciosa‘Hayward’ (kiwifruit) analysed by image cytometry

Autor:	G. C. Lindsay
Rok vydání:	1995
Předmět:	Actinidia deliciosa fungi food and beverages Plant Science Meristem Biology biology.organism_classification Nuclear DNA chemistry.chemical_compound medicine.anatomical_structure chemistry Botany Shoot medicine Feulgen stain Nucleus Cytometry Ecology Evolution Behavior and Systematics DNA
Zdroj:	New Zealand Journal of Botany. 33:243-255
ISSN:	1175-8643 0028-825X
Popis:	Static image cytometry (ICM), computer-enhanced, optical microscopy was used to determine the DNA content of Feulgen-stained nuclei derived from in planta and in vitro shoots of kiwifruit (Actinidia deliciosa var. deliciosa ‘Hayward’). Nuclei in meristem tips, analysed from the same vines, showed alterations in the nuclear DNA content between successive years. These findings correlate closely with flow cytometric analysis of meristem tips of the same vines. The absolute nuclear DNA content for the samples analysed by ICM lay between 5.58 pg DNA per 2C nucleus (spring 1991) and 7.96 pg DNA per 2C nucleus (spring 1992), an increase of 42.5%. The 2C nuclear DNA content of plantlets grown in vitro for 3 years displayed no significant differences, irrespective of the duration on fresh culture medium. The 2C nuclear DNA content of cells from the meristem tips of these plants was approximately 6.71 pg DNA, lower than the high point recorded from in planta apices. These estimates were significantly highe...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6ed48b1830a05e6a964888fa25afe568 https://doi.org/10.1080/0028825x.1995.10410487 Zobrazit plný text záznamu