Effect of Qiguiyin Decoction (芪归银方) on multidrug-resistant Pseudomonas aeruginosa infection in rats

Autor: Qiu Guosong, Wang Lixia, Ling-bo Kong, Bao Yonggang, Qun Ma, Jie Gao, Qing-quan Liu, Zhao Shumin
Rok vydání: 2015
Předmět:
Zdroj: Chinese Journal of Integrative Medicine. 21:916-921
ISSN: 1993-0402
1672-0415
DOI: 10.1007/s11655-015-2089-2
Popis: To investigate the effect of Qiguiyin Decoction (芪归银方, QGYD) on multidrug-resistant Pseudomonas aeruginosa infection in Sprague-Dawley (SD) rats. A pseudomonal infection model in SD rats was established by injecting multidrug-resistant P. aeruginosa intraperitoneally. Infected rats were randomized into four groups treated with Pure water, QGYD, ceftazidime, or combined QGYD and ceftazidime. Blood samples were obtained from the abdominal aorta. Serum was then collected and analyzed by peptide array for immune responsiveness to multidrug-resistant beta-lactamase proteins, including Verona integronen-coded metallo-beta-lactamase 1 (VIM-1), Sao Paulo metallo-beta-lactamase 1 (SPM-1), and Temoniera (TEMs). Blood levels of interleukin-1β (IL-1β), interleukin-4 (IL-4), and interferon-γ (IFN-γ) were assessed by enzyme-linked immunosorbent assay. QGYD enhanced antibody reactivity against VIM-1 [epitopes 7–11 and 36–40] and TEM-1 [epitopes 26–27, 52–55, and 66–70]. QGYD treatment restored the compromised antibody reactivity against VIM-1 [epitopes 53–54 and 56–58] and SPM-1 [epitopes 16–19 and 82–85] following pseudomonal infection. Serum levels of IL-1β and Th1/Th2 in the rats were significantly elevated following pseudomonal infection (P 0.05). QGYD improves the immune response to pseudomonal infection in rats by stimulating the production of protective antibodies against drug-resistant proteins VIM-1, SPM-1, and TEM-1. In addition, it protects the immune system and maintains immune responsiveness by restoring IL-1β and Th1/Th2 levels.
Databáze: OpenAIRE
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