Dye decolorisation by laccase entrapped in copper alginate
| Autor: | Lerluck Chitradon, Churapa Teerapatsakul, Tajalli Keshavarz, Roberto Parra, Christopher Bucke |
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| Rok vydání: | 2007 |
| Předmět: |
Laccase
Copper alginate Chromatography biology Physiology Ganoderma chemistry.chemical_element General Medicine biology.organism_classification Applied Microbiology and Biotechnology Copper Enzyme assay chemistry.chemical_compound chemistry Indigo carmine Residual activity biology.protein Composition (visual arts) Biotechnology |
| Zdroj: | World Journal of Microbiology and Biotechnology. 24:1367-1374 |
| ISSN: | 1573-0972 0959-3993 |
| Popis: | A novel immobilisation system was developed for dye decolorisation using laccase produced by Ganoderma sp. KU-Alk4. The enzyme showed high efficiency in dye decolorisation when entrapped in Cu–Al and Cu-alginate beads. The former gave the highest activity but the enzyme activity survived longer in the latter. An experimental design of two 3 × 3 Latin Square experiments was applied to evaluate the effects of three different alginate compositions (low, intermediate and high mannuronate), concentration of alginate, (1.5, 3.0 and 4.5% w/v) and concentration of cross-linking agent, CuSO4 (0.075, 0.15 and 0.225 M) on the decolorisation of indigo carmine dye and residual laccase activity in beads. The most significant factor for residual activity was the concentration of the cross-linking agent (P |
| Databáze: | OpenAIRE |
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