| Autor: |
Yuki Takakura, Azusa Inoue, Aki Minoda, Taishin Akiyama, Nobuko Akiyama, Takahisa Miyao, Kenta Horie, Yuya Maruyama, Mio Hayama, Tommy Terooatea, Maki Miyauchi, Asako Sakaue-Sawano, Atsushi Miyawaki, Sotaro Hirai, Tatsuya Ishikawa, Takao Seki, Eugene Oh, S. Thomas Kelly, Masaki Yoshida, Masafumi Muratani, Haruka Yabukami, Houko Ohki |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.10.04.463004 |
| Popis: |
SummaryMedullary thymic epithelial cells (mTECs) are critical for self-tolerance induction in T cells via promiscuous expression of tissue-specific antigens (TSAs), which are controlled by transcriptional regulator AIRE. Whereas AIRE-expressing (Aire+) mTECs undergo constant turnover in the adult thymus, mechanisms underlying differentiation of postnatal mTECs remain to be discovered. Integrative analysis of single-cell assays for transposase accessible chromatin (scATAC-seq) and single-cell RNA sequencing (scRNA-seq) suggested the presence of proliferating mTECs with a specific chromatin structure, which express high levels of Aire and co-stimulatory molecules CD80 (Aire+CD80hi). Proliferating Aire+CD80hi mTECs detected by using Fucci technology express a minimal level of Aire-dependent TSAs and are converted into quiescent Aire+CD80hi mTECs expressing high levels of TSAs after a transit amplification. These data provide evidence for the existence of transit amplifying Aire+mTEC precursors during Aire+mTEC differentiation process of the postnatal thymus. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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