Investigation of the interaction of repair DNA polymerase β and autonomous 3′ → 5′-exonucleases TREX1 and TREX2

Autor:	V. M. Krutiakov, N. V. Belyakova, N. L. Ronzhina, O. K. Legina, I. V. Shevelev
Rok vydání:	2010
Předmět:	Real-time polymerase chain reaction DNA clamp biology DNA polymerase DNA polymerase II biology.protein General Agricultural and Biological Sciences Molecular biology DNA polymerase delta DNA polymerase mu General Biochemistry Genetics and Molecular Biology Polymerase Klenow fragment
Zdroj:	Biology Bulletin. 37:464-470
ISSN:	1608-3059 1062-3590
DOI:	10.1134/s1062359010050043
Popis:	The possibility of interaction of recombinant proteins of human repair DNA polymerase β with proofreading 3′ → 5′-exonucleases TREX1 and TREX2 was investigated in vitro for the first time. The results of gel filtration analysis show the formation of a complex between 3′ → 5′-exonucleases mTREX1 and hTREX2 and DNA polymerase β. DNA polymerase activity is shown to increase four-fold in the presence of 3′ → 5′-exonuclease TREX2. The experiments with the use of immunodot and Western blot assays on the binding of DNA-polymerase β with 3′ → 5′-exonucleases TREX1 and TREX2 immobilized on a nitrocellulose membrane provided additional evidence on the direct association of the above proteins in complexes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6eb3e818734df93bfae0690a671f7d75 https://doi.org/10.1134/s1062359010050043 Zobrazit plný text záznamu Plný text ve formátu PDF