Validated Chiral Liquid Chromatographic Method for the Enantiomeric Separation of Florfenicol
Autor: | P. Satyanarayana Rao, Kalpesh Parikh, C. H. S. Prasad, N. Srinivas, T. Joseph Sunder Raj |
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Rok vydání: | 2007 |
Předmět: | |
Zdroj: | Journal of Liquid Chromatography & Related Technologies. 31:231-239 |
ISSN: | 1520-572X 1082-6076 |
DOI: | 10.1080/10826070701738928 |
Popis: | An isocratic normal phase liquid chromatographic (NP‐LC) method was developed for the separation of florfenicol enantiomer in the bulk drug substance. Enantiomeric separation was achieved on a Chiralpack‐AD column (250×4.6 mm) at a constant room temperature using mobile phase combination n‐Hexane:Ethanol:Methanol (80 10 10, v/v/v) delivered at a flow rate of 1.0 mL/min. Analytes were monitored at 225 nm. The method was validated for precision, limit of detection (LOD), limit of quantification (LOQ), linearity, and accuracy. |
Databáze: | OpenAIRE |
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