Validated Chiral Liquid Chromatographic Method for the Enantiomeric Separation of Florfenicol

Autor: P. Satyanarayana Rao, Kalpesh Parikh, C. H. S. Prasad, N. Srinivas, T. Joseph Sunder Raj
Rok vydání: 2007
Předmět:
Zdroj: Journal of Liquid Chromatography & Related Technologies. 31:231-239
ISSN: 1520-572X
1082-6076
DOI: 10.1080/10826070701738928
Popis: An isocratic normal phase liquid chromatographic (NP‐LC) method was developed for the separation of florfenicol enantiomer in the bulk drug substance. Enantiomeric separation was achieved on a Chiralpack‐AD column (250×4.6 mm) at a constant room temperature using mobile phase combination n‐Hexane:Ethanol:Methanol (80 10 10, v/v/v) delivered at a flow rate of 1.0 mL/min. Analytes were monitored at 225 nm. The method was validated for precision, limit of detection (LOD), limit of quantification (LOQ), linearity, and accuracy.
Databáze: OpenAIRE