Morphological analysis of Spirulina sp. NCIM 5143 and Nostoc ellipsosporum NCIM 2786 and comparative characterization of associated enzymes through in silico approach
| Autor: | Shreya Anand, Padmini Padmanabhan, Koel Mukherjee |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cyanobacteria Cyanophycin In silico Microorganism Plant Science Nitrate reductase 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Genetics Molecular Biology Ecology Evolution Behavior and Systematics chemistry.chemical_classification biology Cell Biology biology.organism_classification 030104 developmental biology Enzyme chemistry Nitrogen fixation Animal Science and Zoology Energy source 010606 plant biology & botany |
| Zdroj: | Biologia. 75:1553-1561 |
| ISSN: | 1336-9563 0006-3088 |
| Popis: | Cyanobacteria are the only prokaryotes that can utilize energy sources from sunlight, electrons from water, carbon from the air, and have the capability of fixing nitrogen. The characterization of various cyanobacteria reveals the performance as well as growth differences when compared among different genus of cyanobacteria, which emphasizes the value of characterization in the cellular grounds. In this paper, we have performed a comparative study on the morphology, growth pattern, physiochemical properties as well as structural features of Spirulina sp. NCIM 5143 and Nostoc ellipsosporum NCIM 2786. The comparison is performed through microscopic images, FESEM and statistical study of cell growth by ANOVA. In silico approach was also carried out on the two enzymes present in both the cyanobacteria, Cyanophycin synthestase (EC: 6.3.2.29) and Nitrate reductase (EC: 1.7.1.1) by studying the physicochemical properties, sequential features and structural modeling. A descriptive profile study was completed on these microorganisms and their proteins that may help to interpret the molecular mechanism of the enzymatic reaction of 1, 3-Propanediol (PDO) production in the future. |
| Databáze: | OpenAIRE |
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