Functional expression of mouse insulin-like growth factor-I with food-grade vector in Lactococcus lactis NZ9000
| Autor: | J.-J. Qiao, G. Gao, D.-Z. Jiang, Yanling Bai, Cheng Yang, Xiuming Zhang, Mingqiang Qiao, Ruiqing Li, Haijin Xu, J.-J. Su |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.diagnostic_test
medicine.medical_treatment Growth factor Lactococcus lactis Biological activity Biology biology.organism_classification Applied Microbiology and Biotechnology Molecular biology law.invention Blot Insulin-like growth factor Western blot law medicine Recombinant DNA Polyacrylamide gel electrophoresis |
| Zdroj: | Letters in Applied Microbiology. 54:404-409 |
| ISSN: | 0266-8254 |
| Popis: | Aims: To functionally express the recombinant mouse insulin-like growth factor-I (rtmIGF-I) in Lactococcus lactis NZ9000 with a food-grade vector. Methods and Results: The rtmIGF-I encoding sequence was inserted into secreted food-grade vector pLEB688 and transformed into L. lactis NZ9000. The expression of the recombinant protein rtmIGF-I was confirmed by tricine-SDS-PAGE analysis and Western blot. The concentration of this recombinant protein was 3 mg l−1 in the medium fraction. Further experiment demonstrated that the recombinant protein was biologically active and promoted NIH3T3 cell proliferation in a concentration-dependent manner. Conclusions: The rtmIGF-I was expressed in L. lactis and located into the medium fraction. The optimal final concentration which could promote NIH3T3 cell proliferation after incubation was 100 ng ml−1. Significance and Impact of the Study: The rtmIGF-I was functionally expressed in L. lactis NZ9000 with a food-grade vector. Thus, the recombinant L. lactis NZ9000 could act as a host for the production of rtmIGF-I for further study. The recombinant strain could serve as an IGF-I delivery system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text