| Popis: |
Publisher Summary This chapter outlines the general strategy for the analysis of glycoproteins, utilizing lectin staining, available peptide separation and sequencing techniques, a simple micro-batch affinity method, PDMS, and PMP labeling of carbohydrates. A classical approach of carbohydrate chemists to glycoprotein analysis is to completely deglycosylate the glycoprotein (using hydrazinolysis), isolate and purify the resulting oligosaccharides, and then do structure determinations. Disadvantages to this scheme are that site information is lost and the peptide bonds are usually completely destroyed as well. Advantages are that most protein laboratories have the necessary instrumentation, and both peptide and carbohydrate structural information can be acquired at levels commensurate with the demands of modern protein chemistry. |
