Human Cytomegalovirus Inhibits Granulopoiesis Via Direct Infection and Inducing Apoptosis
| Autor: | Mei Lian Chen, Mo Yang, Xiao Feng Li, Qing Wen Wang, Karen Kwai Har Li, Zheng Xian He, Wei Xiong |
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| Rok vydání: | 2005 |
| Předmět: |
Human cytomegalovirus
medicine.diagnostic_test viruses Immunology Cell Biology Hematology Granulocyte Biology medicine.disease Biochemistry Granulopoiesis Peripheral blood mononuclear cell Virology Molecular biology Flow cytometry medicine.anatomical_structure medicine Bone marrow Annexin A5 Promyelocyte |
| Zdroj: | Blood. 106:3859-3859 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v106.11.3859.3859 |
| Popis: | Human cytomegalovirus (HCMV) infection can cause delayed leukocyte recovery after bone marrow transplantation and often associated with suppression of granulocyte/macrophage progenitor. Leukocyte lineage may be one of the major sites of HCMV infection. However, whether HCMV can interfere with CFU-GM formation and induce apoptosis in granulocyte/macrophage progenitor have not been well investigated. Human bone marrow mononuclear cells (Ficoll), promyelocyte cell line HL-60 and HCMV AD169 strain were co-cultured. Each bone marrow specimen was HCMV DNA and HCMV IgM negative by PCR and ELISA test. Our results showed that HCMV significantly inhibited the formation of CFU-GM as shown in two different concentrations of viral infection groups: 139.26 ± 5.42 (2×105pfu/ml), and 124.19 ± 8.82 (2×106pfu/ml) (colonies/2×105cells/ml, n=26). These were significant differences compared with the blank control group (P |
| Databáze: | OpenAIRE |
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