Sclerotium delphinii causing concentric leaf spots in Piper nigrum in Brazil

Autor: Leandro Jun Soki Shibutani, Enayra Silva Sousa, Maruzanete Pereira de Melo, K. S. Matos, Robinson Severo, José Evando A. Beserra
Rok vydání: 2021
Předmět:
Zdroj: Australasian Plant Pathology. 50:661-670
ISSN: 1448-6032
0815-3191
DOI: 10.1007/s13313-021-00815-y
Popis: Sclerotium delphinii is a common pathogen that causes wilting in a number of plants. In recent years, the genus Sclerotium has been reported as one of those responsible for leaf spots in Piper nigrum in the Amazonian humid tropical regions. In 2017–2018, several black pepper plants presented symptoms of concentric leaf spots. Five isolates were obtained in this study for morphological and phylogenetic characterizations and pathogenicity test. Initially, the gene regions (ITS and LSU) were amplified and sequenced. Phylogenetic analysis confirmed the grouping of the five isolates with the S. delphinii reference isolate (CBS 672.71). The isolates produced sclerotia in all culture media, but some of them altered their capacity to produce sclerotia. The inoculated isolates induced irregular necrotic lesions in the pathogenicity assay using detached leaves. The symptomatic leaves were re-isolated and the fungus culture obtained. No fungus growth was observed in the leaves used as control. This study is the first record of S. delphinii causing leaf spots on P. nigrum in Brazil.
Databáze: OpenAIRE
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