ROS Mediated Cytotoxicity Exhibited by Cashewnut Shell Extract Coated AgNPs Against Staphylococcus aureus Isolated from Milk

Autor: Vellingiri Vadivel, Simran Sinsinwar
Rok vydání: 2020
Předmět:
Zdroj: Journal of Cluster Science. 32:531-547
ISSN: 1572-8862
1040-7278
DOI: 10.1007/s10876-020-01812-9
Popis: This work aimed to synthesize silver nanoparticles (AgNPs) using cashewnut shell extract (CNSE) and evaluate its antibacterial and anti-biofilm activities against Staphylococcus aureus. CNSE showed the presence of isorhamnetin as the major phytochemical compound. The size, shape, morphology and stability of synthesized CNSE-AgNPs were investigated using ultraviolet–visible spectroscopy (peak at 433 nm), zeta potential (− 24.2 mV), field emission scanning electron microscopy (89.1–100.8 nm) and transmission electron microscopy (Spherical shape with diameter of 3–25 nm). X-ray diffraction data indicated the presence of silver in crystalline form (2-theta values of 38, 54 and 77). CNSE-AgNPs showed strong antibacterial activity with zone of inhibition (15 ± 0.06 to 20 ± 0.17 mm) and minimum inhibitory concentration (MIC) (7.81–31.25 μg/ml) against Staphylococcus aureus strains isolated from fresh cow milk samples, methicillin sensitive Staphylococcus aureus (MSSA) (ATCC-25923) and methicillin resistant Staphylococcus aureus (MRSA) (ATCC-700699). CNSE-AgNPs exhibited higher level of nucleotide leakage through membrane disruption (8.97–10.76 folds), reactive oxygen species generation (1.92–3.3 folds), malondialdehyde production (16.85–30.39%) and biofilm inhibition (54.82–78.25%) when compared to untreated Staphylococcus aureus. CNSE-AgNPs was found to effectively eradicate the artificially inoculated Staphylococcus aureus in milk model within 4th h.
Databáze: OpenAIRE
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