Use of PLGA microparticles for vitreous staining during anterior vitrectomy

Autor: Fraj Rose, Dys Chau, Km Shakesheff, M Griffin, Hs Dua, Nl Tint
Rok vydání: 2008
Předmět:
Zdroj: Acta Ophthalmologica. 86
ISSN: 1755-375X
Popis: Purpose To demonstrate in vitro the potential use of FDA-approved PLGA poly(lactic-co-glycolic acid) microparticles to aid visualisation of vitreous during anterior vitrectomy following posterior capsule rupture and to compare with the use of triamcinolone suspension. Methods 10-60um sized PLGA microparticles were fabricated using the single and/or double emulsion technique(s) and used i) untreated (ii) pre-treatment with sodium hydroxide (iii) surface adsorption of a protein/synthetic peptide. Particle size, shape, morphology and surface topography were also assessed using scanning electron microscopy (SEM). The efficacy of these microparticles to enhance visualisation of vitreous against triamcinolone suspension was assessed using a simple in vitro set-up exploiting human cadaveric vitreous. Results Unmodified PLGA displayed a degree of hydrophobicity and also a high rate of dispersion following repeated wash cycles. Interestingly, surface modified PLGA microparticles demonstrated a significant improvement in its 'vitreophilic' properties and were comparable to a triamcinolone suspension. Conclusion The use of modified biodegradable PLGA microparticles may represent a novel method of visualising vitreous and aiding anterior vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous without the pharmacological effects of triamcinolone. Furthermore, such a technique allows the incorporation of drugs, growth factors and/or antibiotics within the microparticles which may be desirable in complicated cataract extraction. Animal models are required to assess in vivo biocompatibility of this method.
Databáze: OpenAIRE
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