High resolution time-domain fluorescence lifetime imaging for biomedical applications

Autor:	M. J. Lever, Anthony K. L. Dymoke-Bradshaw, Mark J. Dayel, S.C.W. Hyde, J. D. Hares, K. Dowling, Paul M. W. French
Rok vydání:	1999
Předmět:	Fluorescence-lifetime imaging microscopy Materials science business.industry High resolution Image intensifier Fluorescence Atomic and Molecular Physics and Optics Imaging phantom law.invention Optics law High temporal resolution Time domain business
Zdroj:	Journal of Modern Optics. 46:199-209
ISSN:	1362-3044 0950-0340
DOI:	10.1080/09500349908231265
Popis:	We report the development of a whole-field fluorescence lifetime imaging (FLIM) system with high temporal resolution based on a time-gated image intensifier. The optimized temporal gate width is < 100 ps, and changes in the environment of a fluorescent phantom, causing lifetime differences less than 10 ps, have been imaged. The versatility of this FLIM system has been demonstrated by measuring both the temporal and spectral profiles of multiple fluorescent samples in a single acquisition. Initial fluorescence lifetime images suggest that this technique can provide a means of distinguishing between different tissue constituents.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6e7545ee64de5717e3769149288c42bd https://doi.org/10.1080/09500349908231265 Zobrazit plný text záznamu