| Autor: |
Senjie Lin, Ling Li, Xiaomei Wu, Yanchun You, Kaidian Zhang, Xin Lin, Jierui Wang, Zhi Zhou, Jiashun Li |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.1101/2020.05.20.107318 |
| Popis: |
Phosphorus (P) is an essential macronutrient for marine phytoplankton responsible for ∼50% of global carbon fixation. As P availability is variable and likely will decrease in future warming oceans, phytoplankton growth will be constrained by their strategies to scavenge dissolved organophosphate. To enhance our mechanistic understanding of these strategies, here we employ CRISPR/Cas9 to create mutants of alkaline phosphatase (AP) PhoA and PhoD and a putative regulator in the diatom model Phaeodactylum tricornutum, coupled with transcriptomic profiling to interrogate their modes of function and P- regulatory network. Results indicate that these two AP isoforms are differentiated in subcellular localization and substrate specialization, and are mutually compensatory and replaceable. Further analyses reveal a regulatory cascade of P scavenging and potential roles of AP in iron and ammonium uptake as well as diverse metabolic pathways. These findings have important implications in how phytoplankton community will respond to future changing microenvironments of global oceans. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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