MDP (Muramyl Dipeptide) Limits Anatomical Damage and Neurological Dysfunction in Rat with Contusive Spinal Cord Injury (SCI) (101.16)

Autor: Maneesh S Garg, Monal Patel, Raisa Puzis, Marcillo E Alexander, Francisco C Pereira, Mary B Bunge, Damien D Pearse
Rok vydání: 2007
Předmět:
Zdroj: The Journal of Immunology. 178:S203-S203
ISSN: 1550-6606
0022-1767
DOI: 10.4049/jimmunol.178.supp.101.16
Popis: MDP is the minimum structural unit responsible for the immunoadjuvancy of bacterial cell wall peptidoglycans. Many properties of MDP manifest potential therapeutic efficacy for SCI. These include stimulation of phagocytosis of immunocompetent cells, thereby facilitating myelin removal with minimal beneficial proinflammatory cytokine alterations,reduced glutamate release,increased intra- and extra-cellular Mg2+, thereby blocking NMDA receptors, andsuppressor cytokine expression, such as IL-10. The current experiment delivered MDP by injection, 6 hours post T8 moderate contusive spinal cord injury in adult Fischer rats. At 2 wk after injury, histological examination revealed reductions in lesion volume associated with deposits of chondroitin sulfate proteoglycan and more myelinated axons. The activated (ED1+) macrophage/microglia count was higher in the more limited injury site of MDP animals. Concurrently, most were Mac1+, indicating active phagocytosis. Furthermore, MDP treated animals displayed fewer ED1+ cells rostral and caudal to the lesion compared with controls. Behaviorally, MDP animals displayed frequent co-ordination with weight-supported steps (BBB, 13.7 ± 1.6) compared with the occasional stepping in controls (BBB, 10 ± 0.6; n=6, p Supported by CRF, Hollfelder Foundation, and Miami Project
Databáze: OpenAIRE
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