| Autor: |
Nur Adilah Abd Rahman, Mohamad Nazib Adon, Radzi Ambar, Chew Chang Choon, Muhammad Mahadi Abdul Jamil, Hassan Buhari Mamman |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Lecture Notes in Electrical Engineering ISBN: 9789811552809 |
| DOI: |
10.1007/978-981-15-5281-6_56 |
| Popis: |
This study is concerned with the investigation of pulsed electric field (PEF) towards biological cells. Biological cells selected in this study are HeLa (cervical cancer) cells. Experimental setup involves different important parts, which include; the source of square wave PEF (ECM®830) that can generate until 3 kV field strength; a modified EC magnetic chamber with incubator system that has been used in order to expose HeLa cells to PEF and a Nikon inverted microscope (Ti-series) for subsequent visualization techniques, image and video. In the early stage, experimental setup was tested by monitoring the proliferation rate of HeLa cells within 0 to 48 h. Then HeLa cells were tested to look at the swelling effect via PEF exposure. After that, we continued to identify the optimum PEF parameters for reversible condition on HeLa cell. As a result, HeLa cells give a good response at 2.7 kV field strength, 30 \( \upmu{\text{s}} \) pulse length with single pulse. Further study showed that two or more adjacent HeLa cells merged together due to increased cell membrane permeability (electrofusion). This discovery triggered an idea to look at the PEF effect on wound healing process. An artificial wound site was investigated with and without PEF exposure. The finding shows that wound area exposed to PEF took 3 h to completely heal while the untreated area took 10 h. This provide a novel technique (electrical based novel treatment) which could be an alternative to drug usage for wound healing process. Overall, the findings achieved in this study could lead us onto a drug free wound healing method. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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