| Autor: |
J. C. Monge, S. Naylor, Keiichi Higuchi, Nathanael J. Lee, A. V. Hospattanker, K. J. Lackner, S. W. Law, S M Grant, Alan Y. Sakaguchi, H. B. BrewerJr., Jeffrey M. Hoeg, Richard E. Gregg |
| Rok vydání: |
1987 |
| Předmět: |
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| Zdroj: |
Cardiovascular Disease ISBN: 9781468452983 |
| DOI: |
10.1007/978-1-4684-5296-9_4 |
| Popis: |
Human liver apo B-100 has been cloned in plasmid and phage λgt-11 expression vectors and apo B-100 cDNA clones were identified by screening with monospecific anti-apo B antibodies and synthetic oligonucleotides based on peptides isolated and sequenced from apo B-100. Overlapping cDNA clones containing the entire apo B-100 mRNA were isolated and sequenced. All peptides previously isolated from apo B-100 mRNA were identified and their locations defined. Northern blot analysis utilizing radiolabeled cDNA probes revealed that the apoB-100 mRNA is 14.1 kb in size. On the contrary, two distinct molecular species of apo B mRNA are being produced by the small intestine. The larger-molecular-weight species migrate at the same position as liver apo B-100 mRNA. The smaller species is 7.5 kb in size, and most likely codes for apo B-48. Hybridization and nucleic acid sequence studies were carried out to define common domains and apo B-100 mRNA-specific domains. Since apo B is a single-copy gene, we propose a mechanism for the generation of apo B-100 mRNA and apo B-48 mRNA through differential splicing of the precursor apo B RNA transcript. We have also localized the apo B gene to the p23 → pter region of the short arm of chromosome 2 by filter hybridization analysis of human-mouse hybrid cell DNAs. We have also initiated studies on the gene organization and expression of apo B in patients with abetalipoproteinemia. Studies on two unrelated kindreds revealed no major deletions or insertions and that the apo B-100 mRNA and apo B-100 protein are present in the liver cells of abetalipoproteinemia patients. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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