Multifunctional crosslinkable itaconic acid copolymers for enzyme immobilization
Autor: | Bernhard Torger, Peter J. Allertz, Frank Simon, Martin Müller, Katrin Salchert, Doris Pospiech, Haike Mäurer, Stefan Keßler, Klaus Jähnichen, Felix Müller |
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Rok vydání: | 2018 |
Předmět: |
chemistry.chemical_classification
Polymers and Plastics Immobilized enzyme Chemistry Comonomer Organic Chemistry technology industry and agriculture General Physics and Astronomy 02 engineering and technology Polymer 010402 general chemistry 021001 nanoscience & nanotechnology Methacrylate 01 natural sciences 0104 chemical sciences Hydrolysis chemistry.chemical_compound Polymer degradation Polymer chemistry Materials Chemistry Copolymer Itaconic acid 0210 nano-technology |
Zdroj: | European Polymer Journal. 102:47-55 |
ISSN: | 0014-3057 |
Popis: | UV-Crosslinkable itaconic copolymers are developed to provide new multifunctional materials for coatings which combine crosslinkable functionalities and the possibility to immobilize enzymes. The polymer-immobilized enzymes were used for water treatment to decompose persistent organic molecules. Introduction of suitable comonomers allows tailoring the mechanical and chemical properties for special applications. Copolymers containing MMA and itaconic anhydride were chosen because of the formation of long-term stable anhydride functionalities. These anhydride functionalities are employed to attach enzymes covalently. 4-Benzoylphenyl methacrylate is used as comonomer for UV-initiated crosslinking. Terpolymers are successfully obtained by radical copolymerization in solution. The copolymers are compared to poly(ethylene-alt-maleic anhydride) [P(EMA)] often used with respect to enzyme immobilization, activity and hydrolytic stability. The hydrolysis stability of the copolymers against water is studied by ATR-FTIR spectroscopy. Thin films are prepared on glass substrates in a layer-by-layer procedure by spin-coating. The layer formation is monitored by ATR-FTIR spectroscopy. UV-crosslinking of the copolymer films is performed taking the optimal irradiation dose that avoids polymer degradation. ATR-FTIR spectroscopy verifies the coupling reaction between amino groups of the enzyme and the anhydride groups on the surface of the crosslinked polymer film. The syringaldazine (4-hydroxy-3,5-dimethoxybenzaldehyde azine) test and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay demonstrate that the immobilized enzymes maintain their activities. The functional copolymers showed a significant effect in reduction of persistent organic pollutants in contaminated waste water. |
Databáze: | OpenAIRE |
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