Biodiagnostics and Polymer Identification with Multiplex Dyes

Autor: Malte Köllner, K.-T. Han, K. Galla, A. Rumphorst, Andreas Schulz, Markus Sauer, K. H. Drexhage, Jutta Arden-Jacob, Jürgen Wolfrum, R. Müller, Stefan Seeger, G. Deltau, G. Bachteler
Rok vydání: 1993
Předmět:
Zdroj: Berichte der Bunsengesellschaft für physikalische Chemie. 97:1542-1547
ISSN: 0005-9021
DOI: 10.1002/bbpc.19930971208
Popis: A new analytical concept based on time-resolved fluorescence spectroscopy is presented, which uses the characteristic fluorescence lifetimes as information to identify new fluorescent dyes, called multiplex dyes. Several dyes can be distinguished at nearly fixed excitation and emission wavelength by recognition of the lifetimes. A pattern recognition technique is used for data analysis. As light sources a pulsed laser diode is used. First experiments are shown in several application fields, like DNA sequencing, immunoassays and tagging of polymer material.
Databáze: OpenAIRE